Shape reconstruction techniques for optical sectioning of arbitrary objects
نویسندگان
چکیده
This paper considers the reconstruction of a shape from an object’s optical sectioning. To this end we describe three algorithms relying on the scalar optics model of light propagation of which the most involved is the deconvolution approach. This approach produces a sequence of deblurred images. We improve the deconvolution approach by suggesting a novel stopping criterion for the iterative process. The performance of the algorithms are illustrated by numerical experiments on microscopic images of biological cells.
منابع مشابه
A Time-Domain Method for Shape Reconstruction of a Target with Known Electrical Properties (RESEARCH NOTE)
This paper uses a method for shape reconstruction of a 2-D homogeneous object with arbitrary geometry and known electrical properties. In this method, the object is illuminated by a Gaussian pulse, modulated with sinusoidal carrier plane wave and the time domains’ footprint signal due to object presence is used for the shape reconstruction. A nonlinear feedback loop is used to minimize the diff...
متن کاملReconstruction and display of curvilinear objects from optical section data using 3-D curve fitting algorithms.
Biological objects resembling filaments are often highly elongated while presenting a small cross-sectional area. Examination of such objects requires acquisition of images from regions large enough to contain entire objects, but at sufficiently high resolution to resolve individual filaments. These requirements complicate the application of conventional optical sectioning and volume reconstruc...
متن کاملQuantification in optical sectioning microscopy: a comparison of some deconvolution algorithms in view of 3D image segmentation
Optical microscopy allows us to study living fluorescent biological samples. Optical sectioning is a technique to obtain three-dimensional (3D) information about the observed object by acquiring a stack of two-dimensional (2D) images at different depths through the sample. However, the specific shape of the 3D optical transfer function of the optical microscope leads to images presenting defect...
متن کاملThree-dimensional reconstruction of the guinea pig inner ear, comparison of OPFOS and light microscopy, applications of 3D reconstruction.
Three-dimensional (3D) reconstruction of anatomical structures can give additional insight into the morphology and function of these structures. We compare 3D reconstructions of the guinea pig inner ear, using light microscopy and orthogonal plane fluorescence optical sectioning microscopy. Applications of 3D reconstruction of the inner ear are further explored. For each method two bullas were ...
متن کاملHybrid method for full identification of buried objects and surrounding media
This study describes a hybrid technique for identification of buried objects. The objects shape and electromagnetic profile are reconstructed from evaluations of electrical permittivity and conductivity. The method suggests a combination of linear sampling and optimization. Linear sampling method (LSM) is used to recover shape and metaheuristic optimizations essential to reconstruct the inside...
متن کامل